Super Resolution Microscope

The second version of the microscope was fitted with eighteen LEDs that allowed for super resolution of the holographic image.  Super resolution is a process by which many low quality images, slightly offset from each other, can be combined to make one higher resolution image.  For a detailed discussion see http://www.photoacute.com/tech/superresolution_faq.html.

 

In practice the microscope was generally used with one LED to view a scene over time.  The fiber optic cables running from each LED act as pinholes.  The videos below show sample reconstructions using a self-programmed GUI in Matlab.  The reconstruction technique was adopted from http://web.mit.edu/optics/www/dhi.htm.

The video below uses the holographic microscopy system to measure the length of a paramecium in two different ways.

The two videos below are both examples of sample reconstructions.

 

The videos were made using a self-created MATLAB GUI.  The GUI takes in the original image, allows the user to crop it, and then select the depth at which the image is reconstructed.  The user can also cycle through time at a particular depth if a series of images was taken.  Below is a screen shot of the GUI.